Table 4.

In vitro assay for estimation of inhibition concentration rate (IC₅₀, μL/mL) for Mentha spp. EOs and their major constituents.

EOs	IC50 a (95% CI b)	Slope ± SD	(χ2) c	p Value
M. citrata	18.295 (12.681–32.359)	0.0787 ± 0.1615	20.597	0.810
M. piperita	0.765 (0.596–0.931)	0.0961 ± 0.3489	9.795	0.711
M. spicata	3.228 (1.780–4.782)	0.1163 ± 0.2551	48.124	0.001
Linalool	0.136 (0.066–0.218)	0.0596 ± 0.656	7.738	0.860
Menthol	76.790 (36.58–166.367)	0.0647 ± 0.093	21.700	0.060
Carvone	1.922 (1.131–3.308)	0.0353 ± 0.3933	12.262	0.506

^a LC₅₀, or Median lethal concentration, is the concentration at which half of the insects died. ^b CI₉₅, Confidence intervals the activity of two compounds was considered significantly different when the 95% CIs failed to overlap. ^c Pearson’s chi-square, suggesting the goodness of fit test, is not significant (ns) at p > 0.05.