Table 4.

Gradient of mobile phases used in the analysis.

	Mobile Phase
Time (min)	A (%)	B (%)
0	100	0
1	100	0
2	100	0
3	95	5
4	95	5
20	70	30
21	50	50
22	50	50
23	50	50
24	20	80
25	20	80
26	0	100
27	0	100
28	100	0
30	100	0