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Effect of carbon source of zwf transcription. P. aeruginosa PAO9011, with zwf::lacZ integrated into the chromosome (single copy), was grown aerobically at 37°C in minimal medium containing various carbon sources: glycerol (40 mM), glucose (20 mM), gluconate (20 mM), lactate (40 mM), fumarate (30 mM), citrate (20 mM), succinate (30 mM), and acetate (60 mM). Cell suspensions were assayed in early log phase for β-galactosidase activity as previously described (45).
