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Analysis of the purification of P. aeruginosa PAO1 G6PDH by SDS-PAGE (10% gel). Lane 1, high-molecular-weight protein standard; lane 2, crude cell extract (45 μg); lane 3, 50% ammonium sulfate cut (30 μg); lane 4, DE-52 column eluate (10 μg); lane 5, hydroxyapatite column eluate, (7 μg); lane 6, Bio-Gel 150 column eluate (2 μg); lane 7, low-molecular-weight protein standard.
