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. 2023 Nov 30;15(23):4980. doi: 10.3390/nu15234980

Figure 7.

Figure 7

Effect of ICZ on model intestinal epithelial cells treated with cytokines. Caco-2 cells plated in 12-well microporous transwells were treated in the basolateral compartment with a cytokine cocktail of IL-1β (50 ng/mL), IFNγ (30 ng/mL), and TNFα (50 ng/mL). mRNA levels normalized to GAPDH of (A) CYP1A1 and (B) IL-8. Changes in permeability were measured by (C) transepithelial electrical resistance (ohms) and (D) mRNA levels of Occludin normalized to GAPDH. Values are mean ± standard error of mean. Data were analyzed by one-way ANOVA; * p < 0.05, ** p < 0.01, **** p < 0.0001.