FIG. 1.
(A) Restriction map of ORF1696 and flanking genes found in an 8-kb region of the photosynthesis gene cluster of the R. capsulatus genome (2). Abbreviations: B, BamHI; Bc, BclI; E, EcoRI; H, HindIII; M, MunI; N, NaeI; P, PstI; St, StuI; X, XhoI. (B) Schematic illustration of ORF1696 and flanking genes with sites of antibiotic resistance cartridge insertion. Solid lines below the genes represent antibiotic resistance cartridge DNA molecules. Stem-loop symbols represent transcriptional terminators. Dashed lines indicate the site of cartridge insertion in ORF1696. The direction of transcription is from left to right, and the puhA promoter (puhAp) is indicated by the bent arrow.