| LRRK2 R1441G knock-in (A549) | Sequences |
|---|---|
| sgRNA | AAGAAGAAGCGCGAGCCUGG |
| Donor sequence | AAATGTGTGCCAACGAGAATCACAGGGGAAGAAGAAGCGCCAGCCTGGAGGGAAAGACACAAAACCCTCTTGTGTTTGCTTTCAAA |
| Forward PCR primer (5'–3') | AGGCATGAAGATGGGAAAGGA |
| Reverse PCR primer (5'–3') | GGAACCCTCGCTTATTCAGGA |
| LRRK2 knock-out (A549) | Sequences |
| sgRNA 1 | GGGGACTGTCGACGGTGATCGGT |
| sgRNA 2 | GGTCCTAAACCTGGTCGCAAAGA |
| Donor sequence | n/a |
| Forward PCR primer (5'–3') | AGTCCGCTGAGTCAGTTTCTTC |
| Reverse PCR primer (5'–3') | GGGCTCTTCATCCCGTTTACA |
| VPS35 D620N knock-in (A549) | Sequences |
| sgRNA | GAUGGCAGCUAGCUGUGCUU |
| Donor sequence | TGTTCACTAGGCATTTTCTCTGTATGAAGATGAAATCAGCAATTCAAAAGCACAGCTAGCTGCCATCACCTTGATCATTGGCACTTTTGA |
| Forward PCR primer (5'–3') | GGCCATGACAACTGATCCCT |
| Reverse PCR primer (5'–3') | GAGAGGGTGCAGCATGTTCT |
| Rab12 knock-out (A549) | Sequences |
| sgRNA | AUCAAACUGUAGAGCUAAG |
| Donor sequence | n/a |
| Forward PCR primer (5'–3') | GGGAGGTTATAGACACTGGTGC |
| Reverse PCR primer (5'–3') | AACTGCTCCCCATGTGCAAG |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.