Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | pT73 Rab10 (rabbit monoclonal) | Denali | 19-4 | WB: 1:500; MSD 1 ug/mL |
| Antibody | pT73 Rab10 (rabbit monoclonal) | Abcam | ab241060 RRID:AB_2884876 |
ICC: 1:100 |
| Antibody | Rab10 (mouse monoclonal) | Abcam | ab104859 RRID:AB_10711207 |
WB: 1:500 |
| Antibody | Rab10 (rabbit monoclonal) | CST | 8127 RRID:AB_10828219 |
ICC: 1:100 |
| Antibody | Rab10 (rabbit monoclonal) | Abcam | ab181367 | MSD: 2 ug/mL |
| Antibody | pS106 Rab12 (rabbit monoclonal) | Abcam | ab256487 RRID:AB_2884880 |
WB: 1:500 |
| Antibody | Rab12 (rabbit polyclonal) | Proteintech | 18843–1-AP RRID:AB_10603469 |
WB: 1:500 |
| Antibody | Rab8a (rabbit monoclonal) | Abcam | ab188574 RRID:AB_2814989 |
WB: 1:500 |
| Antibody | HA (rabbit monoclonal) | CST | 3724 RRID:AB_1549585 |
WB: 1:2000 ICC: 1:100 |
| Antibody | Gal3 (mouse monoclonal) | BD | 556904 RRID:AB_396531 |
WB: 1:500 |
| Antibody | LAMP1 (rabbit monoclonal) | CST | 9091 RRID:AB_2687579 |
WB: 1:500 |
| Antibody | LAMP1 (mouse monoclonal) | Abcam | ab25630 RRID:AB_470708 |
WB: 1:1000 ICC: 1:100 |
| Antibody | LRRK2 (mouse monoclonal) | UC Davis/NIH NeuroMab Facility | N241A/34 RRID:AB_2877351 |
WB: 1:500 |
| Antibody | LRRK2 (mouse monoclonal) | Biolegend | 808201 RRID:AB_2564739 |
MSD: 1 μg/mL |
| Antibody | LRRK2 | Biolegend | 844401 RRID:AB_2565614 |
MSD: 0.5 μg/mL |
| Antibody | pS935 LRRK2 (rabbit monoclonal) | Abcam | ab133450 RRID:AB_2732035 |
WB: 1:500 MSD: 0.5 μg/mL |
| Antibody | GAPDH (mouse monoclonal) | Abcam | ab8245 RRID:AB_2107448 |
WB: 1:500 |
| Antibody | Actin (mouse monoclonal) | Sigma-Aldrich | A2228 RRID:AB_476697 |
WB: 1:5000 |
| Antibody | GM130 (rabbit monoclonal) | Abcam | ab52649 RRID: AB_880266 |
ICC: 1:200 |
| Cell line (Homo sapiens) | HEK293T cells | ATCC | CRL-3216 | |
| Cell line (H. sapiens) | A549 cells | ATCC | CCL-185 | |
| Cell line (H. sapiens) | Rab29 KO A549 cells | Dr. Dario Alessi | ||
| Cell line (H. sapiens) | Rab12 KO A549 cells | Denali | ||
| Cell line (H. sapiens) | LRRK2 R1441G KI A549 cells | Denali | ||
| Cell line (H. sapiens) | VPS35 D620N KI A549 cells | Denali | ||
| Cell line (H. sapiens) | eGFP-LRRK2 HEK293T cells | Denali | ||
| Recombinant DNA reagent | pLVX-TMEM192-3x-HA | Denali | ||
| Recombinant DNA reagent | pcDNA3.1-eGFP-LRRK2 | Denali | ||
| Recombinant DNA reagent | pcDNA3.1-mCherry-Rab12 | Denali | ||
| Chemical compound | LLOMe | Sigma-Aldrich | L7393 | 1 mM |
| Chemical compound | Nocodazole | Sigma-Aldrich | M1404 | 25 μΜ |
| Software, algorithm | Fiji | PMID:29187165 | RRID:SCR_002285 | |
| Software, algorithm | GraphPad Prism | Version 9.5.1 | RRID:SCR_002798 | |
| Software, algorithm | Image Studio Lite | Version 5.2.5 | RRID:SCR_013715 | |
| Software, algorithm | Harmony | Versions 4.9 and 5.1 | RRID:SCR_018809 | |
| Software, algorithm | Python Programming Language | v3.10.8 | RRID:SCR_008394 | |
| Software, algorithm | SciPy | v1.9.3 | RRID:SCR_008058 | |
| Software, algorithm | Napari | v0.4.17 | RRID:SCR_022765 | |
| Software, algorithm | scikit-image | v0.19.3 | RRID:SCR_021142 | |
| Software, algorithm | NumPy | v1.24.2 | RRID:SCR_008633 |