FIG. 6.

Formation of GntR- and CRP-DNA complexes with cleaved gntT DNA fragment. Digestion of the gntT fragment with different restriction enzymes resulted in following fragments: MaeIII (67, 142, and 240 bp), KpnI (182 and 274 bp), and HinfI (223 and 233 bp). The formation of complexes was tested with GntR (A) and CRP (B). Complexes of the DNA fragment with one GntR molecule, two GntR molecules, and Crp were given the suffixes R, R2, and C, respectively.