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Formation of a ternary and a tertiary complex of GntR, CRP, and gntT operator shown by electrophoretic mobility assay. The gntT promoter fragment was incubated with different amounts of CRP and GntR in the presence of 5 mM cAMP. Besides the free DNA (F), three DNA bands with reduced mobility, FC, FCR1, and FCR2, corresponding to DNA bound to one CRP, one CRP and one GntR, and one CRP and two GntR molecules, respectively, were formed.
