TABLE 1.
Bacterial strains, plasmids, and phage used in this study
| Strain, plasmid, or phage | Relevant genotype | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | lacZΔM15 recA | Bethesda Research Laboratories |
| P90C | araΔ(pro-lac) thi | 41 |
| DPB271 | recD::Tn10 F− λ− | B. Bender |
| HT28 | W3110 Δcya::Kanr | 19 |
| RH74 | Δcya-851 ilv::Tn10 | 26 |
| RH77 | Δcya-851 Δcrp zhd-731::Tn10 | 26 |
| HT216 | RH74 gntR::Kanr | This study |
| M15 | recA+ uvr+ F−mtl gal ara lac (pREP4) | 50 |
| NP100 | P90C (λRS88 gntT::lacZ) | 35 |
| PR201 | NP100 gntR::Kanr | This study |
| Plasmids | ||
| pQE-30 | Expression vector; His6 affinity tag | 42 |
| pRS551 | bla′ lacZ+ (operator fusion vector) | 41 |
| pRS552 | bla′ lacZ+ (protein fusion vector) | 41 |
| pTC229 | pTC221 Kanr in StuI | This study |
| pNP-41 | gntR in pQE30 (gntR overexpression) | This study |
| pNP-52 | crp in pQE30 (crp overexpression) | This study |
| Phage λRS88 | bla′ lacZ imm434 ind | 41 |