Fig. 4.

Cellular origin of versican during development of cardiac fibrosis determined by single molecular fluorescence in situ hybridization (smFISH). Representative confocal images of Col1a1 (red), and Vcan (green) in the heart after sham operation (A-C, Control, n = 3) and aortic banding at day 3 (D-F, AB, n = 3) and at day 56 (G-I). DAPI stain for the nuclei (blue). (F) Versican expression by non-cardiomyocytes and cardiomyocytes after 3 days of aortic banding is shown in pink and orange boxes, respectively. The white arrows show positive signal for Col1a1 (A, D, G), green arrows show positive signal for Vcan (E, H), and yellow arrows show colocalization of Col1a1 and Vcan (F, I). Scale bar = 20 μm, 3 μm (insets). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)