TABLE 1.
Primers used
| Name | Sequence (5′-3′) |
|---|---|
| CBPPE | GGAGCGATAACCTTGGAGAAAAG |
| LACZPE | CGACGGGATCTTCGACTCTAGA |
| PUC19F | ATACCGCATCAGGCGCC |
| UCURAT | CCGAATTGTGCAGGTCGACTCTAGAGG |
| URATERMF | ACCTGCACAATTCGGCGTCCCAACTGC |
| SPH1URA | ACATGCATGCGGATGTGCTGTATCGCATCG |
| BAMURAP | CGGGATCCAAGCTTTATATAAACTTGAGAG |
| XBAURAP | GCTCTAGACATTGTAGCAATTCCCGCT |
| CBP1 | CGGGATCCGAACGAACCAATCAGAACAC |
| CBP5 | GCTCTAGACATTTTGAATGACGAAGTGG |
| CBP8 | CGGGATCCGCGTAATGAAAAACGAAGTCG |
| CBP9 | CGGGATCCCCTATTAAATATCGTACAGTA |
| CBP10 | CGGGATCCTACCCCGCCATAGCGAATTG |
| CBP12 | CGGGATCCTTATACTGATGTCTGAACAAT |
| CBPAR | GACATGCACGTAGTCTGGCGGGGTATGACTTCTG |
| CBPAF | GACTACGTGCATGTCTTTCATGTTGCAAGGGCTAA |
| CBPBR | GACATGCACGTAGTCAACCATGAAAGAAGCCAATTC |
| CBPBF | GACTACGTGCATGTCTAATGTTCAACACAAAAGTTC |
| CBPCR | GACATGCACGTAGTCTTGAACATTAAAGTTTAGCCC |
| CBPCF | GACTACGTGCATGTCTAACCAATGGTCACTAACTT |
| CBPDR | GACATGCACGTAGTCCCATTGGTTAACTTGAACTT |
| CBPDF | GACTACGTGCATGTCTCCTATTAAATATCGTACAGT |
| BglII linker | GAAGATCTTC |