Figure 5.
Analysis of the interactions of M0 peptide with liposomes immobilized on the SPR sensor chip
(A) Amino acid sequence of wild type M0 peptide.
(B) Procedure for analyzing the interaction.61 Dodecylamine was coupled with COOH group on the CM5 sensor chip via a conventional amine coupling method. Next, 100 nm LUV solution was injected to be immobilized onto the chip surface. Then the peptide aqueous solution was added to the immobilized liposomes and their interactions were analyzed.
(C) Sensorgrams indicating the interaction of M0 peptide to pure PC, PG/PC (1:9), PA/PC (1:9), and CL/PC (1:9) membranes in acidic buffer (pH 4.0).