REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli BL21(DE3)pLysS	Promega	L1195
Chemicals, peptides, and recombinant proteins
Cardiolipin	Avanti Polar Lipids	Cat#710335P, CAS: 115404-77-8
Phosphatidylglycerol (DOPG)	Avanti Polar Lipids	Cat#840475P, CAS: 67254-28-8
Phosphatic acid (DOPA)	Avanti Polar Lipids	Cat#840875P, CAS: 108392-02-5
Phosphatidylcholine (DOPC)	Avanti Polar Lipids	Cat#850375P, CAS: 4235-95-4
6-mercaptohexanoic acid	Sigma-Aldrich	Cat#674974, CAS: 17689-17-7
6-mercaptohexanol	Sigma-Aldrich	Cat#725226, CAS: 1633-78-9
Succinic acid	Fujifilm-Wako	Cat#194-04335, CAS: 110-15-6
HEPES	Fujifilm-Wako	Cat#346-01373, CAS: 7365-45-9
KCl	Fujifilm-Wako	Cat#163-03545, CAS: 7447-40-7
EDTA	Fujifilm-Wako	Cat#345-01865, CAS: 6381-92-6
Tween 20	TCI	Cat#T2530, CAS: 9005-64-5
Dodecylamine	Sigma-Aldrich	Cat#325163-5ML, CAS: 124-22-1
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide	TCI	Cat#D1601, CAS: 25952-53-8
N-hydrosuccinimide	TCI	Cat#H0623, CAS: 6066-82-6
HBS-EP buffer	Cytiva	Cat#BR100188
Tetrabutylammonium chloride	TCI	Cat#T0055, CAAS 1113-67-0
Phosphatidylcholine (POPC)	Avanti Polar Lipids	Cat#850457, CAS: 26853-31-6
HBS-N buffer	Cytiva	Cat#BR100828
DMSO	Fujifilm-Wako	Cat#043-07216, CAS: 67-68-5
Ethanolamine-HCl	TCI	Cat#A0298, CAS:2002-24-6
NaOH	Fujifilm-Wako	Cat#194-18865, CAS: 1310-73-2
2-Propanol	Fujifilm-Wako	Cat#161-09163, CAS: 67-63-0
SDS	Fujifilm-Wako	Cat#196-08675, CAS: 151-21-3
fl-KcsA	Iwamoto et al.56	N/A
ΔM0-KcsA	Iwamoto and Oiki34	N/A
M0 peptide	GenScript	N/A
n-dodecyl-β-D-maltoside	Dojindo	Cat#D316, CAS: 69227-93-6
Succinic acid	Nacalai	Cat#32402-05, CAS: 110-15-6
Isopropyl β-d-thiogalactopyranoside	Nacalai	Cat#19742-36, CAS:367-93-1
Potassium phosphate buffer	Promega	V446A-C
2-mercaptoethanol	Nacalai	Cat#21417-52, CAS:60-24-2
Imidazole	Nacalai	Cat#19004-35, CAS:288-32-4
Critical commercial assays
CM5 sensor chip	Cytiva	Cat#BR100530
SIA Kit Au	Cytiva	Cat#BR100405
Recombinant DNA
pET29c(+)	EMD Biosciences	N/A
Software and algorithms
BiaEvaluation software	Cytiva	N/A
AFMViewer version 220418 software	This paper	N/A
pCLAMP software	Molecular Devices	Ver. 10.7
BiaEvaluation software	Cytiva	N/A
Other
Biacore T100	Cytiva	N/A
AFM system	Yamamoto70	N/A
Cantilever	Olympus	BL-AC10DS-A2
Contact bubble bilayer system	Iwamoto and Oiki50	N/A
Patch-clamp amplifier	Molecular Devices	Axopatch 200B
Analog-to-digital converter	Molecular Devices	Digidata 1550A
Plasma cleaner	Harrick Plasma	PDC-32G
Scanning electron microscope	Hitachi High-Tech	S-4100