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. 2023 Nov 13;25(12):1774–1786. doi: 10.1038/s41556-023-01280-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Percentage of dead cells for the indicated cell lines treated with NAMPTi or NAD⁺ as indicated for 72 h (BJ and BJ-T) or 48 h (HeLa and U2OS). Data are smoothed conditional means and CI95. Biological replicates, n = 18 (non-treated conditions) and n = 6 (all other conditions) except for BJ-T (non-treated = 12). b, Immunoblot confirmation of mitochondrial (mito.) isolation from HeLa cells treated for 24 h without (NT) or with NAD⁺ as indicated or 10 nM NAMPTi. The experiment was performed twice with similar results. c, Mitochondrial NAD(H) quantification in HeLa cells treated without (NT) or as indicated with NAD⁺ or NR. Data are mean+SD, n = 3 biological replicates, two-tailed Student’s t-test. d-e, Relative ATP levels (d) and cell number (e) of HeLa cells treated without (NT) or with NAD⁺ or NR as indicated. Data are mean+SD, n = 6 biological replicates, two-tailed Welch’s t-test. Numerical data and uncropped immunoblots are available in Source Data Files.

Source data