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. 2023 Nov 13;25(12):1774–1786. doi: 10.1038/s41556-023-01280-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, All metabolic parameters from mitochondrial stress analysis relative to non-treated (NT) cells. The indicated cell lines were treated with 2 mM NAD⁺ for the indicated time. Data are mean+SD, n = 5 (BJ-T) and n = 6 (HeLa and U2OS) independent experiments, two-tailed Welch’s t-test. OCR, oxygen consumption rate; MRR, maximum respiratory rate; ECAR, extracellular acidification rate. b, Flow cytometry analysis of mitochondrial membrane potential using the TMRM cell-permeant dye. HeLa cells were treated without (NT) or with 2 mM of NAD⁺ (left panel) or NR (right panel) for the indicated time, or with 10 µM FCCP for 30 min. The same data for NT and FCCP conditions are presented in both panels. c, Flow cytometry analysis of EdU incorporation and cell cycle in HeLa cells treated for 24 h without (NT) or with 5 mM pyruvate, 2 mM NAD⁺, and/or 20 mM aspartate. Flow cytometry experiments were performed twice with similar results. Numerical data are available in Source Data Files.

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