Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 13;25(12):1774–1786. doi: 10.1038/s41556-023-01280-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, Basal OCR and ECAR for the indicated cell lines. Data are mean + s.d. Independent experiments, n = 5 (BJ-T) and n = 6 (all other cell lines). b, Metabolic parameters from mitochondrial stress analysis relative to non-treated (NT) cells. HeLa and U2OS cells were treated with 2 mM NAD⁺ as indicated. Data are mean + s.d., n = 6 independent experiments, two-tailed Welch’s t-test. MRR, maximum respiratory rate. c,d, QIBC analysis of mitochondrial membrane potential in HeLa (c) and U2OS (d) cells using TMRM and MTG cell-permeant dyes. The ratios of TMRM and MTG intensities are normalized to non-treated (NT) cells. Cells were treated for 24 h with 2 mM NAD⁺, 5 µM oligomycin (Oligom), 10 µM BPTES and/or 10 µM FCCP. Cells quantified in c: NT, 1,570; NAD⁺, 923; Oligom, 2,107; NAD⁺ + Oligom, 1,518; BPTES, 1,274; NAD⁺ + BPTES, 1,258; FCCP, 1,261; NAD⁺ + FCCP, 1,330. Cells quantified in d: NT, 1,177; NAD⁺, 2,025; Oligom, 821; NAD⁺ + Oligom, 808; BPTES, 635; NAD⁺ + BPTES, 1,029; FCCP, 634; NAD⁺ + FCCP, 462. Data are from a representative experiment performed twice with similar results. Two-tailed Welch’s t-test. e, QIBC analysis of γH2AX foci, EdU intensity and DNA content in HeLa and U2OS cells treated for 24 h without (NT) or with NAD⁺ as indicated and/or 10 µM FCCP. Representative experiment from two independent experiments. For boxplots in c and d, the centre line indicates the median; box limits indicate the 25th and 75th percentiles; minima and maxima of whiskers extend 1.5 times the inter-quartile range from 25th and 75th percentiles, respectively. Numerical data are available in source data files.

Source data