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. 2023 Nov 9;25(12):1833–1847. doi: 10.1038/s41556-023-01281-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Immunofluorescence showing the nuclear localization of AF1 and AF2 ER domains through the detection of the 6x His-tag. Scale bar, 20 μm. b, PLA of HA or 6x His-tag antibody alone or both antibodies together in MAF-overexpressing MDA-MB-231 cells transduced with AF1 or AF2 ER domains. Representative confocal microscopy images for PLA red signal and DAPI nuclear staining with magnified inset are shown. Scale bar, 50 μm; inset scale bar, 10 μm. c, PLA quantification. Each dot represents the average PLA signal from 64 to 208 nuclei per condition. n = 3 biological replicates. Bars represent mean ± sem. Statistical significance, two-tailed Wilcoxon rank-sum test.

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