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. 2023 Dec 4;25(12):1821–1832. doi: 10.1038/s41556-023-01274-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — (a) 30 pathways most enriched in differentially abundant genes (log₂(fold change) ≥ 1) in multipotent basal cells and basal-derived luminal cells identified by KEGG pathway analysis. Metabolism-related pathways highlighted in green (enriched in multipotent basal-enriched) and blue (enriched in basal-derived luminal-enriched). (b) Intracellular flow cytometry analysis of the basal marker cytokeratin 5 (KRT5) and the luminal marker cytokeratin 8 (KRT8) in primary basal-derived mouse organoids three, six and nine days after plating into organoid culture. (c) Principal component analysis of metabolic profiling data for basal-derived organoids with three technical replicates per timepoint. (d) Heatmap of metabolite abundance in primary basal-derived mouse organoids (n = 3 independent biological replicates per timepoint). (e) Fractional contribution from [U-¹³C]glucose to M2 and M3 citrate in basal-derived organoids (n = 3 independent biological replicates per timepoint). Error bars represent SEM. (f) Western blot analysis of proliferation marker PCNA in basal-derived organoids.

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