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. 2023 Oct 9;19(12):1916–1926. doi: 10.1038/s41567-023-02218-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, Toy simulations studying the polarity-sorting kinetics of filaments with varying intrinsic curvatures, lengths and bending rigidities—snapshot of the initial (left) and final (right) configuration of FtsZ L169R filaments at a high density of ϕ = 0.88. The properties of the mutant substantially slow down the polar alignment of filaments in all the studied packing fractions. Only bonds of the filaments (without the full diameter of beads) are presented for clarity. Filaments are colour coded according to the orientation of the bond vectors between beads. b, Fraction of the parallel (polar) alignment of filaments as a function of simulation time. The FtsZ L169R was simulated with two times higher persistence and length than WT, no intrinsic curvature and eight times lower v₀ (parameters v₀ = 3.625 d/τ, Pe = 300, ℱ = 20, L_fL169R = 16d, dθ = 0 rad, ϕ = 0.22–0.88). Although FtsZ WT prefers to align in a parallel orientation, FtsZ L169R stays aligned in a nematic fashion until the end of simulations. c, Representative TIRF micrographs of Alexa488-FtsZ L169R at increasing FtsZ L169R and constant FtsA concentration. We observe very static thread-like self-organization of filaments with no concentration-dependent transitions. The micrographs are representatives of at least four independent experiments (n = 6, 10, 6, 8 and 4 for [FtsZ] = 0.625, 1.15, 1.25, 1.5 and 3 µM). d, Snapshots of large-scale FtsZ L169R simulations with increasing density. The FtsZ L169R filaments were modelled with altered properties according to the HS-AFM analysis: filaments were two times longer and more rigid, had no intrinsic curvature and v₀ was eight times lower, resulting in three times lower Peclet number and two times lower flexure number than FtsZ WT (parameters v₀= 3.625 d/τ, Pe = 300, ℱ = 20, dθ = 0 rad, L_fL169R = 16d). FtsZ L169R exhibits a static thread-like pattern in all the densities, as in the experiment. The filaments are colour coded according to the orientation of the bond vectors between beads.

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