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. 2023 Nov 27;25(12):1746–1757. doi: 10.1038/s41556-023-01284-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — (a-c) The expression patterns of Adrb1 (a), Adrb2 (b), and Adrb3 (c) (which encode the β1, β2, and β3 adrenergic receptors, respectively) among non-hematopoietic bone marrow cells based on single cell RNA sequencing from ref. ²⁹. (d-h) Adrb2 (d), Adrb3 (e), Scf (f), Vegf (g), and Ang2 (h) transcript levels by qRT-PCR in unfractionated cells, LepR⁺ cells, and adipocytes from adult mouse bone marrow 14 days after irradiation and transplantation of a radioprotective dose of bone marrow cells (a total of 3 mice (for unfractionated cells and LepR⁺ cells) or 5 mice (for adipocytes) from 3 independent experiments). All data represent mean ± standard deviation. (i-l) Generation of Adrb2 ^flox and Adrb3 ^flox mouse alleles. (i) The Adrb2 ^flox allele was generated by inserting loxp elements on either side of exon 1. The insertion sites were chosen to avoid disrupting intron sequences that are conserved among species. Using the Adrb2 ^flox allele, Cre recombination removed exon 1, which contains the entire Adrb2 coding sequence. (j) PCR genotyping of genomic DNA with the primers shown in panel i confirmed germline transmission of the Adrb2^flox allele (representative of three independent experiments). Mice were backcrossed at least five times onto a C57BL/Ka background before analysis. (k) The Adrb2 ^flox allele was generated by inserting loxp elements on either side of exon 2. The insertion sites were chosen to avoid disrupting intron sequences conserved among species. Cre recombination removed exon 2, which contains the start codon, generating a frameshift mutation. (l) PCR genotyping of genomic DNA with the primers shown in panel k confirmed germline transmission of the Adrb3^flox allele (representative of three independent experiments for j-l). Mice were backcrossed at least five times onto a C57BL/Ka background before analysis. All data represent mean ± standard deviation.

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