TABLE 3.
Complementation of SVM167
| Strain | CHO
|
HEp-2
|
||
|---|---|---|---|---|
| % Invasiona | % of wild-type level | % Invasion | % of wild-type level | |
| 14028s (wild type) | 4.8 ± 0.3 | 100 | 5.3 ± 0.3 | 100 |
| 14028s (pWKS30) | 2.3 ± 0.0 | 100 | 5.7 ± 0.8 | 100 |
| 14028s (pHH20) | 2.2 ± 0.05 | 100 | 3.3 ± 0.2 | 100 |
| 14028s (pHH26) | 4.5 ± 0.15 | 100 | 7.8 ± 0.1 | 100 |
| SVM167 (sigE::Tn10dTc) | 0.3 ± 0.07 | 6.3 | 0.53 ± 0.06 | 10 |
| SVM167 (pWKS30) | 0.2 ± 0.02 | 8.7 | 0.4 ± 0.07 | 7 |
| SVM167 (pHH20) | 0.8 ± 0.05 | 36 | 1.6 ± 0.3 | 49 |
| SVM167 (pHH26) | 1.5 ± 0.2 | 33 | 2.3 ± 0.02 | 35 |
a
Percentage of intracellular bacteria ± range after allowing bacteria to invade during a 1-h incubation; duplicate assays were performed. Values are representative of several assays done in this study.