Fig. 2.

Adgrl1-deficient mice develop obesity. (a, b) Male global Adgrl1 KO mice have higher body weight (a) and exhibit hyperphagia (b) (at 12 weeks of age). (c) Adgrl1 gene KO was validated by qRT-PCR in the hypothalamus. Adgrl1 KO mice and their littermate WT mice were euthanised at 29 weeks of age for qRT-PCR. (d, e) Adgrl1 knockdown in the VMH (Adgrl1^VMH KD) was induced in 8-week-old male and female mice. (d) Body weight in male Adgrl1^VMH KD mice and littermate controls was measured at different times; n=15 Ctrl and n=25 Adgrl1^VMH KD mice. (e) Body weight in female Adgrl1^VMH KD mice and their littermate controls; ovariectomy in corresponding groups was performed 1 week after inducing the Adgrl1 deficiency; n=10 Ctrl and n=8 Adgrl1^VMH KD mice. Data are shown as means ± SEM. Two-tailed Student’s unpaired t test or repeated measures ANOVA (1- or 2-way) followed by Bonferroni multiple comparison test: *p<0.05, ***p<0.001 vs Ctrl, over the indicated time points. Ctrl, control; Ovx, ovariectomy/ovariectomised mice