Fig. 3.

Impaired feeding responses in male Adgrl1^VMH KD (knockdown of Adgrl1 in the VMH) mice and effect of fasting on Adgrl1^VMH expression. (a, b) Feeding responses to oral (a) or intra-VMH (b) glucose administration (500 mg glucose in 300 µl water, oral gavage, or 5 mmol/l glucose in 2 µl PBS, intra-VMH, administered at 16:00 and 17:00 hours for both) in the 3rd week following Adgrl1^VMH deficiency in 11-week-old Adgrl1^VMH KD mice and their littermate controls; n=6 or 8 Ctrl and Adgrl1^VMH KD mice. Two-tailed Student’s t test: **p<0.01 vs corresponding group without glucose. (c) Feeding response following overnight (18:00 to 09:00 hours) fasting in the 3rd week after Adgrl1^VMH deficiency in 11-week-old Adgrl1^VMH KD mice and their littermate controls; n=8 Ctrl and Adgrl1^VMH KD mice. (d) Overnight (18:00 to 09:00 hours) fasting reduces Adgrl1 mRNA levels (measured by qRT-PCR) in the VMH of 8-week-old C57BL/6 male mice. Two-tailed Student’s unpaired t test or repeated measures two-way ANOVA followed by Bonferroni multiple comparison tests: **p<0.01 vs all other groups at corresponding times. ^†p<0.05, ^††p<0.01 vs Adgrl1^VMH KD (non-fasted) at the corresponding time. Ctrl, control; Glu, glucose