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. 2023 Oct 13;59(9):684–696. doi: 10.1007/s11626-023-00803-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Figure 4. — Effect of EA on ECM in HK-2 cells. (A) Expression of EMT-related gene mRNA E-cadherin (a), vimentin (b), α-SMA (c), in HK-2 cells. (B) The mRNA level of Col-1 in HK-2 cells under different concentrations of EA. (C) Cell activity of HK-2 cells at different concentrations of EA. (D) mRNA expression of ECM protein CTGF (a), collagen Ι (b), collagen Ш (c), in HK-2 cells. (E) Protein expression of ECM (a) in HK-2 cells and quantification of extracellular matrix protein FN (b), collagen Ш (c), collagen Ι (d). Values are the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control group; #p < 0.05, ##p < 0.01, ### p < 0.001, vs. TGFβ1 group.