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Steady-state kinetic parameters of recombinant G6PD enzymes.
| Construct | Amino acid change | kcat (s-1) | KmNADP+ (µM) | KmG6P (µM) |
|---|---|---|---|---|
| G6PD WT | - | 326 | 12.9 ± 2.9 | 46.1 ± 3.8 |
| G6PD Meyer | Arg219Gly | 6 | 18.1 ± 2.7 | 49.5 ± 6.5 |
Experiments were performed in triplicate, and data are presented as mean ± SD.
