TABLE 1.
E. coli K-12 strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strainsa | ||
| GS162 | Wild type | This lab |
| GS597 | metJ97 | This lab |
| GS1029 | Δ(gcvA):ΣaadA | 26 |
| GS1053 | gcvR::Tn10 | 8 |
| GS1066 | recD::Tn10 | This lab |
| GS1111 | Δ(gcvR bcp)Σneo | This study |
| Plasmids | ||
| pGS311 | Single-copy cloning vector | 8 |
| pGS334 | Multicopy gcvR+ plasmid | 8 |
| pGS338 | Single-copy gcvR+ plasmid | 8 |
| pGS343 | gcvR-lacZ fusion plasmid | This study |
| pGS393 | pGS343 with -13A gcvR promoter mutation | This study |
| pGS394 | pGS343 with -36A gcvR promoter mutation | This study |
| pGS429 | pGS334 with -13A gcvR promoter mutation | This study |
| pGS430 | bcp-lacZ fusion plasmid | This study |
| pGS431 | pGS430 with -13A gcvR promoter mutation | This study |
| pGS433 | pBR322 with SspI site replaced by SmaI site | This study |
| pGS434 | Multicopy gcvR+ bcp+ plasmid | This study |
| pGS437 | Δ(gcvR bcp)Σneo in pGS434 | This study |
| pGS451 | pGS437 with extra 2 kb of homologous DNA upstream of the Δ(gcvR bcp)Σneo | This study |
a
All also carry thi, pheA905, ΔlacU169, araD129, and rpsL150 mutants.