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Expression of a gcvR-lacZ fusion from wild-type and mutant gcvR promoters
| Strain | β-Gal activity (Miller units)a
|
||
|---|---|---|---|
| GM | Glycine | Inosine | |
| GS162λgcvR-lacZ | 73 | 78 | 69 |
| GS162λgcvR-13A-lacZ | 5 | 5 | 4 |
| GS162λgcvR-36A-lacZ | 7 | 8 | 7 |
Cells were grown in GM medium with supplements as indicated and assayed for β-Gal activity. Each value is the average of two separate determinations, each performed in triplicate. All standard deviations are ≤11% of the mean.
