TABLE 4.
Bcp is not involved in the regulation of gcvT-lacZ expression
Strain | Relevant genotype | β-Gal activity (Miller units)a
|
||
---|---|---|---|---|
GM | Glycine | Inosine | ||
GS162λgcvT-lacZ[pGS311] | Wild type | 159 | 1020 | 15 |
GS1053λgcvT-lacZ[pGS311] | gcvR | 1,443 | 1,710 | 1,012 |
GS1111λgcvT-lacZ[pGS311] | gcvR bcp | 1,400 | 1,559 | 909 |
GS1111λgcvT-lacZ[pGS338] | gcvR bcp[gcvR+] | 205 | 1,150 | 16 |
Cells were grown in GM medium with supplements as indicated and assayed for β-Gal activity. Each value is the average of two separate determinations, each performed in triplicate. All standard deviations are ≤12% of the mean.