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. 2023 Dec 8;14:8121. doi: 10.1038/s41467-023-43751-9

Fig. 8. SENP3 or SENP5 deficiency triggers CDK6 loss and cell cycle arrest in BxPC3 and Ramos cell lines.

Fig. 8

a BxPC3 cells were transfected with siRNAs as indicated. 72 h after transfection cells were lysed and proteins were analyzed by immunoblotting as indicated. b Cell cycle analysis by PI staining of BxPC3 cells depleted for SENP3 (red), SENP5 (blue), SENP3/5 (green) or siControl (black) for 72 h. Percentage of cells in the G1, S or G2 phase was determined and significance level was calculated using two-sided t-testing. Experiments were performed with two replicates and are shown as mean ± standard deviation. c Depletion of SENP3 or SENP5 with two different siRNAs in Ramos cells for 72 h. Cell lysates were analyzed by immunoblotting as indicated. d PI staining of Ramos cells transfected with two independent siRNAs against SENP3 (orange, red), SENP5 (blue, green) or siControl (black). Percentage of cells in G1, S or G2 phase was determined and significance level was determined by two-sided t-testing. Experiments were performed with three replicates and are shown as mean ± standard deviation. Source data for (a)–(d) are provided as a Source Data file.