Fig. 6. Experimental validation of the core inflammation program on the protein level.

a Inflammation-specific protein-coding genes were identified by filtering the genes depicted in Fig. 3c for the surfaceome as previously described⁴⁵. Genes that encode proteins selected for validation (based on antibody availability and panel design) are labeled in red. b Experimental overview. Human and mouse neutrophils were isolated from peripheral blood or bone marrow, respectively, cultured 48 h, and analyzed in flow cytometry. c Flow cytometry analysis of resting and activated mouse and human neutrophils. The gating strategy is depicted in Supplementary Fig. 11. Significance indicates adjusted P-values of a Dunn’s test (two-sided) that followed a Kruskal-Wallis H test (significant for all markers). Exact P-values are provided in the Source Data file. d Top, diffusion map embedding of neutrophils from humans and mice cultured for 48 h with or without GM-CSF + LPS and GM-CSF + IFN-γ. Diffusion map embedding calculated based on CD69, CD14, IL-4R, and PD-L1. Bottom, diffusion map embedding of human and mouse neutrophils, colored by species. e Diffusion map embedding colored by marker expression highlights a continuum driven by increasing expression of the activation markers. Source data are provided as a Source Data file.