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Confirmation of purified proteasome species using SDS-PAGE
(A) To confirm whether PSMD14-HTBH transduction was successful, SDS-PAGE analysis followed by immunoblotting was performed using both the 6× His tag and PSMD14 antibodies.
(B) The purified proteasome species from A549-PSMD14-HTBH cells were confirmed through CBB staining.
(C) The purified proteasomes were also confirmed through immunoblotting.
