Table 3.
Parameters used for the operation of the mass spectrometer
| Parameter | Value |
|---|---|
| Polarity | positive |
| Global settings | |
| Methods Duration (min) | 160 |
| Ion Source Type | NSI |
| Spray Voltage: Positive Ion (V) | 1800 |
| Ion Transfer Tube Temp (°C) | 290 |
| Default Charge State | 2 |
| Full MS Scans | |
| Mass analyzer | Orbitrap |
| Automatic Gain Control (AGC) target | 300% |
| Microscans | 1 |
| Orbitrap resolution | 120,000 |
| Scan range (m/z) | 350–1,800 |
| Max Injection Time (ms) | 25 |
| AGC Target | Custom |
| DataType | Profile |
| Filter Settings | |
| Include Charge State (s) | 2–6 |
| Exclusion Duration (s) | 35 |
| Mass tolerance (ppm) | 10 |
| Dependent MS/MS Scans | |
| Mass analyzer | Orbitrap |
| Automatic Gain Control (AGC) target | 100,000 |
| Microscans | 1 |
| Activation type | HCD |
| Normalized collision energy (%) | 30 |
| Isolation Window | 1.2 |
| First Mass | 110 |
| Number of dependent scans | 20 (from most intense precursors) |
| Scan rate | Normal |
| Mass range | Normal |
| Charge exclusion | Exclude +1 charge state |
| Isolation Mode | Quadrupole |
| Ion trap scan rate | Rapid |
| Max Injection Time (ms) | Custom |
| Resolution | 15000 |
| Maximum ion time | 25 ms |
| Loop count | 10 |
| Isolation offset | 0 |