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Unicorn7 software settings
| Parameter | Value |
|---|---|
| Equilibration | |
| Flow rate | 0.2 mL/min |
| Inlet | A |
| Total volume | 2.0 CV |
| Reset UV monitor | |
| Sample application | |
| Flow rate | 0.2 mL/min |
| Loop type | Capillary loop |
| Empty loop | 1 mL |
| Fractionate state | In waste (do not collect) |
| Elution | |
| Flow rate | 0.2 mL/min |
| Inlet | A |
| Isocratic elution volume | 1.50 CV |
| Fractionate state | fraction collector |
| Fraction type | fixed volume fractionation |
| Fixed fractionation volume | 0.25 mL |
