Denaturation buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| DTT | 0.1 M | 0.01543 g |
| Tris-HCl pH 8.5 (1 M) | 0.1 M | 100 μL |
| 10% Sodium dodecyl sulfate (SDS) | 2% | 200 μL |
| water (HPLC grade) | N/A | 700 μL |
| Total | 1 mL |
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