Urea buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Urea | 8 M | 4.8048 g |
| Tris-HCl, pH 8.5 (1 M) | 0.1 M | 1 mL |
| water (HPLC grade) | N/A | Up to 10 mL |
| Total | 10 mL |
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