Protease inhibitor cocktail (PIC; 100×)
| Reagent | Final concentration | Amount |
|---|---|---|
| Aprotinin (in ddH2O) | 80 μM | 2.6 mg |
| Leupeptin (in ddH2O) | 2 mM | 4.8 mg |
| Pepstatin A (in DMSO) | 1 mM | 3.43 mg |
| DMSO | N/A | Up to 5 mL |
| Total | 5 mL |
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