Reagent | Final concentration | Amount |
---|---|---|
Novex Tris-Glycine Native Running Buffer (10×) | 1× | 20 mL |
EDTA (0.5 M) | 0.1 mM | 40 μL |
ATP (250 mM) | 0.5 mM | 0.4 mL |
MgCl2 (1 M) | 5 mM | 1 mL |
DTT (1 M) | 0.5 mM | 0.1 mL |
ddH2O | N/A | 178.46 mL |
Total | 200 mL |
Reagent | Final concentration | Amount |
---|---|---|
Novex Tris-Glycine Native Running Buffer (10×) | 1× | 20 mL |
EDTA (0.5 M) | 0.1 mM | 40 μL |
ATP (250 mM) | 0.5 mM | 0.4 mL |
MgCl2 (1 M) | 5 mM | 1 mL |
DTT (1 M) | 0.5 mM | 0.1 mL |
ddH2O | N/A | 178.46 mL |
Total | 200 mL |