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. 2023 Dec 8;14:8142. doi: 10.1038/s41467-023-43672-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a DCF-DA and (b) AmplexRed determination of ROS dynamics in HepG2 (15 min DMSO n = 50; 15 min IFT: n = 18; 24 h DMSO: n = 17; 24 h IFT: n = 11 independent cell samples per condition) (15 min: p < 0.0001); 24 h: p < 0.0001) and C. elegans (15 min: n = 4; 48 h: n = 3 biologically independent samples per condition) (15 min: p = 0.018; 48 h: p = 0.002 for 1 nM and p = 0.0003 for 10 nM), respectively. c NRF2 luciferase reporter activation in cells overexpressing human catalase (CAT Oex) upon isofalcarintriol (1a) treatment (10 µM) compared to empty vector control (n = 10 independent cell samples per condition) (p < 0.0001). d Lifespan of ctl-1 (cytosolic catalase)-overexpressing nematodes. e Paraquat stress assay (10 mM paraquat) of C. elegans treated with isofalcarintriol (1a) (1 nM) compared to DMSO control. f Paralysis assay with GMC101 nematodes, a protein aggregation model of Alzheimer’s disease upon Isofalcarintriol (1a) treatment. g Motility assay with AM23 (control strain) and AM176 (CAG repeat strain) nematodes, as a protein aggregation model of Huntington’s disease (AM23 DMSO: n = 277; AM23 IFT: n = 258; AM716 DMSO: n = 276; AM716 IFT: n = 277 nematodes per condition in two independent experiments, p < 0.0001 for each comparison). h Proliferation assay with MCF-7 and its non-tumor control cells, HMEpC (MCF-7 0.1 and 50 µM: n = 6; MCF-7 1 and 10 µM: n = 3; HMEpC: n = 3 independent cell samples per condition) after 96 h treatment with of isofalcarintriol (1a) (0.1 µM: p = 0.01; 1 µM: p < 0.0001, 10 µM: p = 0.0006, 50 µM: p = 0.0005). i Soft agar colony formation assay. j MCF-7 (p = 0.05) (DMSO: n = 8; IFT: n = 9 independent cell samples per condition), k HepG2 (p < 0.0001) (n = 9 independent cell samples per condition), and l HT-29 (p < 0.0001) when treated with isofalcarintriol (1a) (DMSO: n = 6; IFT: n = 8 independent cell samples per condition). Scale bar: 1 mm. Data are represented as average or average ± SD or + SD. Data shown in (a), (b), (h), (i), and (j) were measured in ≥ 3 independent experiments. Statistics: log-rank test, two-sided unpaired student’s t-test, one-way ANOVA and Dunnett’s posthoc test, two-way ANOVA and Sidak’s post-hoc test. Source data are provided as a Source Data file.