Fig. 2.

Multiple histopathological profiles of DMG H3-K27 with BRAF or FGFR1 mutations. Case 14 a A glioneuronal proliferation with ganglion cells, eosinophilic granular bodies and some microcalcifications (HPS, magnification × 400). Case 11 b A glioneuronal proliferation with numerous ganglion cells (HPS, magnification × 400). Case 13 c A glioneuronal proliferation with numerous ganglion cells and lymphocytic infiltrates (HPS, magnification × 400). Case 31 d A mainly circumscribed proliferation (neurofilament, magnification × 30). Case 32 e A mainly circumscribed proliferation (neurofilament, magnification × 400) with a diffuse component at the periphery of the tumour f (neurofilament, magnification × 400). g Diffuse chromogranin A immunoreactivity staining neuron cells (magnification × 400). h BRAF^V600E expression in all tumour cells including ganglion cells (magnification × 400). i H3K27M expression in all tumour cells including ganglion cells (magnification × 400). Case 7 j A glial proliferation with oligo-like features and microcalcifications (magnification × 400). Case 11 k Global loss of H3K27me3 (magnification × 400). (l) Loss of ATRX in tumour cells (magnification × 400). m Whorls of gliofibrillary processes (HPS, magnification × 20). n Whorls of gliofibrillary processes (HPS, magnification × 400), stained using GFAP antibody o, magnification × 400). Black scale bars represent 50 μm (a–c, e–l and n–o), 100 µm (m), and 500 µm (d)