TABLE 1.
E. coli K-12 strains and plasmids
| Strain or plasmida | Genotype or descriptionb | Source or reference |
|---|---|---|
| Strains | ||
| DH5α | F− φ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) supE44 relA1 deoRΔ(lacZYAargF) U169 | Lab stock |
| JC7623 | recB21 recC22 sbc-15 ara arg his leu pro thr | A. J. Clark (23) |
| JM109 | endA1 recA1 gyrA96 thi hsdR17 (rK− mK+) relA1 supE44 Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15] | Promega, Inc. |
| NU426 | W3110 prototroph [W3110 sup(Am); probably W1485E] | C. Yanofsky (2) |
| NU402 | NU426 pdxB::ΩKanr | Lab stock (1) |
| NU816 | W3110 tnaA2 ΔlacU169 | C. Yanofsky (2) |
| NU1018 | NU814 pdxB::mini-Tn10Tetr | Lab stock used in cross |
| TX2768 | NU816 pdxH::ΩCmr | Lab stock (26) |
| TX3632 | NU426 pdxB::ΩKanrpdxK::mini-Tn10Cmr | Lab stock (44) |
| TX3634 | NU426 pdxB::ΩKanrpdxK::mini-Tn10Cmr | NU402 × P1vir(TX3632) |
| TX3689 | NU426 pdxK::mini-Tn10Cmr | NU426 × P1vir(TX3632) |
| TX4015 | NU426 pdxB::mini-Tn10Tetr | NU426 × P1vir(NU1018) |
| TX4016 | NU426 pdxB::mini-Tn10TetrpdxK::mini-Tn10Cmr | TX3634 × P1vir(NU1018) |
| TX4017 | JC7623 pdxY::ΩKanr | JC7623 × linear pTX623 |
| TX4021 | NU426 pdxY::ΩKanr | NU426 × P1vir(TX4017) |
| TX4022 | NU426 pdxB::mini-Tn10TetrpdxY::ΩKanr | TX4015 × P1vir(TX4017) |
| TX4023 | NU426 pdxK::mini-Tn10CmrpdxY::ΩKanr | TX3689 × P1vir(TX4017) |
| TX4024 | NU426 pdxB::mini-Tn10TetrpdxK::mini-Tn10CmrpdxY::ΩKanr | TX4016 × P1vir(TX4017) |
| Plasmids | ||
| pHP45-ΩKanr | Plasmid carrying ΩKanr cassette | P. Prentki and H. M. Krisch (30) |
| pTX303 | 0.57-kb AvaI-SacII fragment from pTX281 cloned into the BamHI site of pGEM3Z; source of RNA probes 1 and 1o | Lab stock (24) |
| pTX485 | pdxK+ clone in pGEM3Z vector; pdxK transcription direction opposite to that of lacZ | Lab stock (44) |
| pTX608 | 1.1-kb minimal pdxY+ clone in pUC19 vector; pdxY transcription in same orientation as lacZ | This work |
| pTX618 | 1.6-kb pdxY+ clone in pUC19 vector; pdxY transcription in same orientation as lacZ | This work |
| pTX623 | pTX618 pdxY::ΩKanr | This work |
| pTX628 | 1.5-kb AvaI-EcoRV fragment from pNU217 (24) cloned into AvaI and HincII sites of pGEM3Z; source of RNA probes 2 and 2o | This work |
| pTX629 | 0.60-kb EcoRI-PstI fragment from pTX630 cloned into EcoRI and PstI sites of pGEM3Z; source of RNA probes 5 and 5o | This work |
| pTX630 | 1.6-kb pdxY+ clone in pGEM3Z vector; source of RNA probes 4 and 4o | This work |
| pTX632 | Self-ligation of 2.7-kb fragment from the AvaI and BamHI double digestion of pTX628; source of RNA probes 3 and 3o | This work |
a
See Materials and Methods for additional details about strain and plasmid constructions.
b
Kanr, Tetr, and Cmr, resistance to kanamycin, tetracycline, and chloramphenicol, respectively. Besides the antibiotic resistances indicated, all plasmids also imparted resistance to ampicillin (Apr).