TABLE 3.

Growth properties of pdxB, pdxK, and pdxY single, double, and triple mutants on supplemented MMG^a

Strain	Supplement added to MMG
	None	PN	PL	GA	4HT
NU426 (parent)	+	+	+	+	+
TX3689 (pdxK)	+	+	+	+	+
TX4021 (pdxY)	+	+	+	+	+
TX4023 (pdxK pdxY)	+	+	+	+	+
TX4015 (pdxB)	−	+	+	+	+
TX4016 (pdxB pdxK)	−	−	+	+	+
TX4022 (pdxB pdxY)	−	+	+	+	+
TX4024 (pdxB pdxK pdxY)	−	−	−	+	+

^aBacteria were streaked from LB plates containing 1 mM GA and appropriate antibiotics onto freshly prepared MMG plates containing the indicated supplements. Plates were incubated at 37°C for about 40 h before growth was scored. Supplements were added at the following final concentrations: PN, 1 μM; PL, 1 μM; GA, 1 mM; 4HT, 6 μM. Commercial PM is only about 98% pure and gave the same results as PL (data not shown). +, confluent growth in heavily streaked areas and formation of single colonies; −, no single-colony formation.