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Steady-state mRNA levels of GLZ16 and GLZ22 determined by Northern blot analysis. GLZ16 and GLZ22 are isogenic constructs that differ only in their amounts of gene 60 sequence between the landing site and the junction with lacZ (Table 1). Test, test construct; Δgap, gap deletion construct. Amounts of lacZ mRNA per lane were normalized to the amount of bla mRNA per lane. The data shown represent results of a single experiment (see the text).
