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. 1998 Apr;180(7):1822–1830. doi: 10.1128/jb.180.7.1822-1830.1998

TABLE 1.

Summary of gene 60-reporter fusionsa

Plasmid Promoter RBSb 5′ additionc No. of codons after landing sited U of activity withe:
Avg bypassing efficiency ± SD (%)f
Test fusion Δgap fusion
BX3* tac UAGAGGGUAUUAUG AUGAAAAGCUUA(AUG) 2 6,107 ± 490 24,270 ± 2,540 24.7 ± 1.4
pSK31 tac Same as for BX3* GST 7 4,432 ± 85 31,648 ± 1,956 14.0 ± 0.8
pPG33 tac Same as for BX3* T7 gene 10 7 5,042 ± 120 42,156 ± 1,895 12.0 ± 0.5
pPA31 tac Same as for BX3* ProtA-CAT 7 11,484 ± 590 49,852 ± 1,890 23.0 ± 1.4
NH11 λ pLpR bla signal peptide 24 81 ± 6 216 ± 12 37 ± 3
GLZ16 Consensus UCAGGAAGCUUAUG 2 140 ± 3 2,273 ± 27 6.2 ± 0.1
GLZ22 Consensus Same as for GLZ16 24 99 ± 5 960 ± 28 10.3 ± 0.3
GLZ30 tac Same as for GLZ16 24 7,010 ± 1,400 18,580 ± 1,170 37 ± 4
GLZ32 Consensus UCCGGAAGCUUAUG 24 69 ± 2 706 ± 20 9.8 ± 0.3
GLZ34 Consensus UGAGGAAGCUUAUG 24 209 ± 16 1,320 ± 60 15.9 ± 0.6
GLZ40 Consensus AUGAGGUGUAUAAUG 24 207 ± 24 1,370 ± 35 15.2 ± 1.4
GLZ49 Consensus UAGGAGGAAGCUUAUG 24 131 ± 5 919 ± 45 14.3 ± 0.2
pGG34 tac CAGGAAACAGAAGCUUAUG 2 19,220 ± 3,540 72,450 ± 6,232 26 ± 5
pGG36 lacUV5 Same as for pGG34 2 4,250 ± 820 23,620 ± 2,750 18 ± 4
pGG45 tac Same as for pGG34 7 10,940 ± 950 52,080 ± 3,820 21 ± 2
pGG47 tac Same as for pGG34 24 14,200 ± 1,620 50,640 ± 4,520 28 ± 4
a

All reporter fusions were made to lacZ except that with NH11, which was made to phoA. 

b

The underlined region is the SD region of the ribosome-binding sequence (RBS); the boldface region is the initiation codon in each construct. 

c

In the sequence, the initiation codon is in boldface type, and the beginning of the gene 60 coding sequence is in parentheses. T7 gene 10, 5′ fragment of T7 gene 10; ProtA, immunoglobin-binding domain from protein A. BX3* carries two transversions at nt 87 and 89 that create an internal BglII site and that do not affect bypassing efficiency. 

d

Number of gene 60 codons between the landing site and reporter fusion. 

e

Activities are expressed in β-Gal units, except for that for NH11, which is expressed in phosphatase units. Values are averages of multiple independent measurements. Δgap, gap deletion. 

f

Values are average efficiency estimates from several independent experiments.