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Affinities of PBPs in β-lactam-resistant transformants of S. pneumoniae R6. Cell lysates of the transformants were incubated with the relative [3H]propionylampicillin concentrations (in microcuries) indicated at the top. PBPs of the transformants are indicated to the left. (Top) R6T1-P with a low-affinity PBP2b; (middle) R6T2-CC with a low-affinity PBP2x; (bottom) R6T4-CCCB (all high-Mr PBPs are low-affinity variants and only the low-Mr PBP3 is labeled at all concentrations).
