TABLE 1.
Strains used in this studya
| Strain | Genotype | Source or reference | Text designation |
|---|---|---|---|
| E. coli | |||
| AMS150 | K-12 rpoS::Tn10 (Tcr) | 18 | |
| MG1655 | λ− F−; prototroph | 13 | Wild type |
| CF022 | MG1655 rpoN208::Tn10 (Tcr) | MG1655 × YMC18 | RpoN− |
| CF023 | MG1655 rpoS::Tn10 (Tcr) | MG1655 × AMS150 | RpoS− |
| CF029 | MG1655 glnG10::Tn5 (Kmr) | MG1655 × LR1 | NtrC− |
| CF030 | MG1655 glnD99::Tn10 (Tcr) | MG1655 × RB9040 | Utase/UR− |
| CF1652 | MG1655 ΔrelA251 | M. Cashel | RelA− |
| LR1 | lacIqlacL8 glnG10::Tn5/λgln105 (Kmr) | 21 | |
| NR2 | MG1655 phoB::Tn5 (Apr) | 20 | PhoB− |
| RB9040 | endA1 thi-1 hsdR17 supE44 hutCKlebs Δ(lac)U169 glnD99::Tn10; Mu lysogen (Tcr) | 3 | |
| YMC18 | endA1 thi-1 hsdR17 supE44 hutCKlebs Δ(lac)U169 rpoN208::Tn10 (Tcr) | 24 | |
| CF5802 | MG1655 Δppk Δppx::kan (Kmr) | 13 | PPK− PPX− |
| Other | |||
| P. aeruginosa PAO1 | A. Chakrabarty | ||
| A. johnsonii | ATCC 17909 |
a
Strains constructed for this study were made by standard P1vir transduction methods in which MG1655 was the recipient.