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. 2023 Dec 8;21:891. doi: 10.1186/s12967-023-04524-6

Table 3.

Degenerate primers designed for the subcloning of the selected CD19-specific VHHs into the pET-26b(+) expression vector

Designation Sequence
Forward-NcoI-Degenerate 5′—CATGCCATGGCCATGSAGGTSCAGCTGCWGG—′3
Reverse-XhoI-Degenerate 5′—CCGCTCGAGAAGATCTTCTTCGCTAATAAGTTTTTGTTCKGAGSWSACKGTSACC-3′