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RNAscope ISH results, illustrating the cellular distributions of CB1Rs in the midbrain ventral tegmental area. CB1 mRNA was detected in GAD1-labeled GABAergic neurons (A), VgluT2-labeled glutamate neurons (B) and a small population of TH-labeled DA neurons (C) in the midbrain of WT, but not CB1-KO mice (D).
