Table 2.

Summary of cannabinoid (THC, WIN55,212-2, JWH133)-induced tetrad effects in transgenic animals.

Transgenic mouse line	Analgesia	Hypothermia	Catalepsy	Hypoactivity	References
Wildtype	Normal THC or WIN effect	Normal effect	Normal effect	Normal effect	Monory et al. (2007) and Wang et al. (2020)
Global CB1-KO	No effect (to THC or WIN)	No effect	No effect	No effect	Ledent et al. (1999), Monory et al. (2007), Wang et al. (2020), and Zimmer, Zimmer, Hohmann, Herkenham, and Bonner (1999)
CaMK-CB1-KO (${\text{CB}}_1^{\text{flox}}$ xCaMK-Cre)	↓ THC effect	No effect	No effect	No effect	Monory et al. (2007)
Glu-CB1-KO (${\text{CB}}_1^{\text{flox}}$ xNEX-Cre)	Normal THC effect	↓ THC effect	Normal effect	↓ THC effect	Monory et al. (2007)
GABA-CB1-KO (${\text{CB}}_1^{\text{flox}}$ xDlx5/6-Cre)	Normal THC effect	Normal effect	Normal effect	Normal effect	Monory et al. (2007)
D1-CB1-KO (${\text{CB}}_1^{\text{flox}}$ xDrd1-Cre)	Normal THC effect	↓ THC effect	↓ THC effect	Normal effect	Monory et al. (2007)
SNS-CB1-KO (${\text{CB}}_1^{\text{flox}}$ xNav1.8-Cre)	↓ WIN effect	N/D	Normal effect	N/D	Agarwal et al. (2007)
Global CB2-KO	↓ THC or WIN effect	Normal effect	↓ THC or WIN effect	Normal effect (rotarod) ↓ THC effect (open-field)	Wang et al. (2020) and Li et al. (2021)
DA-CB2-KO (${\text{CB}}_2^{\text{flox}}$ xDAT-Cre)	Normal effect (to WIN)	Normal effect	↓ WIN effect	Normal effect	Liu et al. (2017))
CX3CR1-CB2-KO (${\text{CB}}_2^{\text{flox}}$ xCX3CR1-Cre)	Normal effect (to WIN, ACEA)	Normal effect	Normal effect	Normal effect	Liu, Canseco-Alba, Liang, Ishiguro, and Onaivi (2020)
Immune-CB2-KO (CB2flox xLysM-Cre)	Normal JWH133 effect	N/D	N/D	N/D	Cabañero et al. (2020)
Syn-CB2-KO (${\text{CB}}_2^{\text{flox}}$ xSyn-Cre)	↓ JWH133 effect	N/D	N/D	N/D	Cabañero et al. (2020)
SNS-CB2-KO (${\text{CB}}_2^{\text{flox}}$ xNav1.8-Cre)	↓ JWH133 effect in thermal pain	N/D	N/D	N/D	Cabañero et al. (2020)
GPR55-KO	Normal THC effect ↑ WIN effect	↑ THC effect	↑ THC effect	↑ THC effect	Wang et al. (2020)

Abbreviations: CaMK—Ca⁺⁺/calmodulin-dependent protein kinase II; CB1^flox—CB1-floxed mice; NEX—a gene encodes neuronal helix-loop-helix protein (a transcription factor), expressed in pyramidal neurons of the dorsal telencephalon during embryonic development; Dlx5/6—distal-less homeobox genes 5/6, expressed in forebrain GABAergic neurons during embryotic development; SNS—sensory neurons expressing Nav1.8 channel; DA—dopamine; LysM—lymphocytes/monocytes; Syn—Synapsin (a neuronal marker).